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Title:                                       Dr. Mrs.

Name:                                     Nancy Odurowah Quashie (aka Duah)

Academic Qualifications:      BSc, MPhil, PhD

Professional Qualification:   Parasitologist

Email address:                       nduah@noguchi.ug.edu.gh; nduah@yahoo.com

Telephone (office):                +233 302 501178/9 ext 1210

Fax number:                          +233 302 502182

Department:                          Epidemiology

 

Short Bios/CV:                

My research areas include Parasitology, Molecular Biology and Immunology. I have been working on Antimalarial Drug Resistance for over 17 years in Prof. Kwadwo Koram’s malaria research group at the Noguchi Memorial Institute for Medical Research (NMIMR). The group has been working in collaboration with the National Malaria Control Programme (NMCP) for the monitoring of antimalaria drug efficacy in Ghana since 1998. I am proud to state that the change in antimalarial drug policy in Ghana in 2005 was based on the scientific evidence-based data from clinical/in vivo,in vitro and molecular analysis conducted by our group. I have been in charge of the molecular analysis in characterising markers of ant malarial drug resistance for the group. My major collaborators are research scientists from the US Naval Medical Research Unit (NAMRU) with funding from the Global Emerging Infections Surveillance and Response System (GEIS) to characterise molecular markers of drug resistance. I also collaborate with Ventachalam Udhayakumar and his group from the Centers for Disease Prevention and Control (CDC) for molecular analysis of drug resistance. In addition to antimalarial drug resistance studies, I have worked on the determinants of antibody responses to malaria vaccine candidate antigens because of the observed variation in host immune responses to these antigens. My main collaborator is Prof. David Conway, London School of Hugiene and Tropical Medicine (LSHTM)

 

Research Interest:

·         Host Parasite Interactions of Infectious Tropical Diseases

·         Malaria Parasitology, Epidemiology and Immunology

·         Antimalarial Drug Resistance

·         Human Population Movements and Transmission of Infectious Tropical Diseases

 

Research Projects completed within the past five (5) years:

1.      Capacity building to prepare West African sites for clinical trials on HIV, TB and Malaria. West African Network of Excellence for TB, AIDS and Malaria (WANETAM) (2010 to 2015).

Funding agency: European and Developing Countries Clinical Trials Partnership (EDCTP)

2.      Characterization of molecular markers of Plasmodium falciparum antimalarial drug resistance from ten regions of Ghana (2009 to 2013).

Funding agency: US Naval Medical Research Unit (NAMRU)/ Global Emerging Infections Surveillance and Response System (GEIS)

3.      Surveillance for antimalarial drug resistance in Ghana (2011 to 2012)

Funding agency: NAMRU/ GEIS

 

On-going Research Projects:

1.      Title: Characterization of the K-13 molecular marker of Plasmodium falciparum artemisinin resistance: a retrospective study.

Goal: The study will characterise the K-13 marker in archived samples collected over 15 years from sentinel sites across Ghana and also detect any evolutionary trends and signatures of positive selection in the gene.

Role: Principal investigator (PI) in charge of all aspects of the study

Funding agency: US Naval Medical Research Unit (NAMRU)/ Global Emerging Infections Surveillance and Response System (GEIS)

 

2.      Title: Surveillance of antimalarial drug resistance in Ghana

Goal: The general objective of this study is to continue with the assessment of therapeutic efficacy and safety of artesunate-amodiaquine and artemether-lumefantrine for the treatment of uncomplicated P. falciparum malaria in three selected sentinel sites in the three ecological zones of Ghana using in vivo, in vitro and molecular methods

Role: Co-investigator in charge of the molecular analysis of drug resistance

Funding agency: US Naval Medical Research Unit (NAMRU)/ Global Emerging Infections Surveillance and Response System (GEIS)

 

3.      Title: Monitoring of the efficacy of antimalarial drugs for the treatment of uncomplicated malaria in Ghana

Goal: This study involves the assessment of the therapeutic efficacy and safety of artesunate-amodiaquine and artemether-lumefantrine (first line drugs) for the treatment of uncomplicated P. falciparum malaria in 10 sentinel sites throughout Ghana. The assessment will include clinical, parasitological, in vitro and molecular methods. The findings will help formulate recommendations for the Ministry of Health make informed decisions on updating the current antimalarial treatment guidelines.

Role: Co-investigator in charge of molecular analysis of drug resistance

Funding agency:Global Fund for TB, Aids and Malaria/National Malaria Control Program

 

4.      Title: Tracking malaria prevalence in 30 districts in Ghana

Goal: This surveillance study is assessing the progress of interventions towards reduction of malaria prevalence in Ghana. The assessment will include the generation of data on malaria parasite positivity rates across Ghana by monitoring malaria parasite positivity rates using Rapid Diagnostic Tests (RDTs) and microscopy in 20 selected health facilities across Ghana.

Role: Co-investigator in charge of molecular analysis for parasite speciation and genetic diversity

Funding agency: Global Fund for TB, Aids and Malaria/National Malaria Control Program

 

 

List of Publications

 

ARTICLES:

 

1.      Atelu, G. R.,. Duah, N. O. and Wilson, M. D. Carriage of sub-microscopic sexual and asexual Plasmodium falciparum stages in the dry season at Navrongo, Ghana  Ghana Medical Journal 2016 Manuscript ID GMJ-2016-04-0049 (submitted under review)

2.      Duah, N. O., Matrevi, S. A., Quashie, N. B., Abuaku, B. and Koram, K. A. Genetic diversity of Plasmodium falciparum isolates from uncomplicated malaria cases in Ghana over a decade. Parasites & Vectors2016 9:416. doi: 10.1186/s13071-016-1692-1

3.      Adika, C.,Aboagye-Antwi,F., Appiah-Opong,R.,Duah, N. 0., Ayanful-Torgby,R.,Matrevi, S.,Osei-Safo,·D.,· Gyan, B.A., Quashie N.B.Assessment oftheeffectsofnaturalcocoaextracton erythrocytemembraneandasexualerythrocyticstageof Plasmodiumfalciparum. Asian Journal of. Ethnopharmacology andMedicinalFoods,2016. 02{02);11-16.

4.      Asare KK, Boampong JN, Duah NO, Afoakwah R, Sehgal R, Quashie NB.Synergism between Pfcrt and Pfmdr1 genes could account for the slow recovery of chloroquine sensitive Plasmodium falciparum strains in Ghana after chloroquine withdrawal.Journal of Infections and Public Health. 2016. doi: 10.1016/j.jiph.2016.02.004.

5.      Bushman M, Morton L, Duah N, Quashie N, Abuaku B, Koram KA, Dimbu PR, Plucinski M, Gutman J, Lyaruu P, Kachur SP, de Roode JC, Udhayakumar V. Within-host competition and drug resistance in the human malaria parasite Plasmodium falciparum.Proceedings of the Royal Society B. 2016 283(1826).  doi: 10.1098/rspb.2015.3038

6.      Abuaku B, Duah N, Quaye L, Quashie N, Malm K, Bart-Plange C, Koram K. Therapeutic efficacy of artesunate-amodiaquine and artemether-lumefantrine combinations in the treatment of uncomplicated malaria in two ecological zones in Ghana.Malaria Journal2016 15(1):6. doi: 10.1186/s12936-015-1080-x.

7.      Dery V, Duah NO, Ayanful-Torgby R, Matrevi SA, Anto F, Quashie NB. An improved SYBR Green-1-based fluorescence method for the routine monitoring of Plasmodium falciparum resistance to anti-malarial drugs.Malaria Journal2015 14(1):481. doi: 10.1186/s12936-015-1011-x.

8.      Quashie,N. B.,Duah, N. O., Abuaku, B., Quaye, L., Ayanful, R., Akwoviah, G. A., Kweku, M., Duplessis, C., Johnson, J., Lucchi, N. W., Udhayakumar, V., Kronmann, K. C., Koram, K. A. A SYBR Green-based in vitro test of susceptibility of Ghanaian Plasmodium falciparum clinical isolates to a panel of antimalarial drugs. Malaria Journal2013 12:450.

9.      Bushman, M.,Duah, N. O., Quashie, N., Abuaku, B., Koram, K. A., de Roode, J., Udhayakumar, V.Within-host competition of malaria parasites in humans and the fitness cost of drug resistance. American Journal of Tropical Medicine and Hygiene2013 89 (5):155. .

10.  Duah, N.O., Matrevi, S.A., de Souza, D.K., Binnah, D.D., Onwona, C. O., Tamakloe, M. M., Opoku, V.S., Quashie, N. B., Abuaku, B., Duplessis, C., Kronmann, K.C., Koram, K.A. Increased pfmdr1 gene copy number and a decline in pfcrt and pfmdr1 resistance alleles in Ghanaian Plasmodium falciparum isolates after the change of antimalarial drug treatment policy. Malaria Journal2013 12:377.

11.  Enweronu-Laryea, C.C., Adjei, G.O., Mensah, B., Duah, N., Quashie, N.B. Prevalence of congenital malaria in high-risk Ghanaian newborns: a cross-sectional study.Malaria Journal2013 12:17.

12.  Duah, N.O., Quashie, N.B., Abuaku B., Sebeny, P., Kronmann, K. and Koram, K.A. Surveillance of molecular markers of sulphadoxine-pyrimethamine resistance in Ghana after the change of malaria treatment policy. American Journal of Tropical Medicine and Hygiene2012 87(6):996-1003.

13.  Abuaku, B., Duah, N., Quaye, L., Quashie, N., Koram, K. Therapeutic efficacy of artemether-lumefantrine combination in the treatment of uncomplicated malaria among children under five years of age in three ecological zones in Ghana.Malaria Journal2012 11:388.

14.  Duah, N. O., Abuaku, B., Quashie, N., Kronmann, K., Koram, K. Surveillance of molecular markers of sulphadoxine-pyrimethamine resistance in Ghana after the change of malaria treatment policy American Journal of Tropical Medicine and Hygiene2011 85(6):201.

15.  Kweku, M. A., Appiah, E., Duah, N. O., Koram, K. A. Greenwood, B., Chandramohan, D. Assessment of the efficacy, tolerability and ease of administration of dihydroarthemisinin plus piperaquine and artesunate plus sulfamethoxypyrazine plus pyrimethamine compared with sulphadoxine-pyrimethamine for preventing malaria in Ghanaian children. American Journal of Tropical Medicine and Hygiene2011 85(6):139.

16.  Alam, T., de Souza, D. K., Vinayak, S., Griffing, S. M., Poe, A. C., Duah, N. O., Ghansah, A., Asamoa, K., Slutsker, L., Wilson, M. D., Barnwell, J. W., Udhayakumar, V., Koram, K A., Selective sweeps and genetic lineages of Plasmodium falciparum drug resistant alleles in Ghana. Journal of Infectious Diseases 2011, 203:220-227.

17.  Holder, B.S., Miles D.J.C., Kaye S., Crozier S., Duah N.O., Roberts E., Ojuola O.O., Palmero, Touray E.S., Waight P., Cotten M., Rowland-Jones S., van der Sande M. and Whittle H.C. Epstein-Barr virus but not Cytomegalovirus is associated with reduced vaccine antibody responses in Gambian infants. PLoS One2010, 5(11):e14013.

18.  Duah, N.O., Miles, D.J.C., Whittle, H.C., Conway, D.J. Acquisition of antibody isotypes against Plasmodium falciparum blood stage antigens in a birth cohort. Parasite Immunology2010 32(2):125-34.

19.  Duah, N.O., Weiss, H.A., Jepson, A., Tetteh, K.K., Whittle, H.C., Conway, D.J. Heritability of antibody isotype and subclass responses to Plasmodium falciparum antigens. PLoS One2009 4(10):e7381.

20.  Ceesay, S.J., Casals-Pascual C., Erskine J., Anya S.E., Duah N.O., Fulford A.J., Sesay S.S., Abubakar I., Dunyo S., Sey O., Palmer A., Fofana M., Corrah T., Bojang K.A., Whittle H.C., Greenwood B.M., Conway D.J. Changes in malaria indices between 1999 and 2007 in The Gambia: a retrospective analysis.Lancet2008 372(9649):1545-1554.

21.  Akpogheneta, O.J., Duah N.O., Tetteh K.K., Dunyo S., Lanar D.E., Pinder M., Conway D.J. Duration of naturally acquired antibody responses to blood-stage Plasmodiumfalciparum is age dependent and antigen specific.Infection and Immunity2008 76(4):1748-1755.

22.  Duah, N.O., Tetteh, K., Akpogheneta, O., Polley, S., Corran, P., Whittle, H. and Conway, D. J. The rate of acquisition of antibody isotype profiles to Plasmodium falciparum blood stage antigens in Gambia infants. American Journal of Tropical Medicine and Hygiene2007 77(5): 85-86.

23.  Quashie N.B., Duah N.O., Abuaku B., Koram K.A. The in-vitro susceptibilities of Ghanaian Plasmodiumfalciparum to antimalarial drugs.Annals of Tropical Medicine and Parasitology. 2007 101(5):391-398.

24.  Duah, N.O., Wilson M.D., Ghansah A., Abuaku B., Edoh D., Quashie N.B., Koram K.A. Mutations in Plasmodiumfalciparum chloroquine resistance transporter and multidrug resistance genes, and treatment outcomes in Ghanaian children with uncomplicated malaria.Journal of Tropical Pediatrics. 2007 53(1):27-31.

25.  Koram, K.A., Abuaku B., Duah N., Quashie N. Comparative efficacy of antimalarial drugs including ACTs in the treatment of uncomplicated malaria among children under 5 years in Ghana.Acta Tropica. 2005 95(3):194-203.

26.  Quashie, N.B., Ofori-Adjei D., Duah N.O. and Koram, K. In vitro susceptibility of Plasmodium falciparum isolates chloroquine and other antimalarial drugs in Ghana. Ghana Medical Journal2004 38, 131-135.

27.  Duah, N.O., Ghansah A., Koram K., Edoh D., Boakye D. A. and Wilson, M. D. Studies on genetic mutations in Plasmodium falciparum strains associated with 4-aminoquinolines (Chloroquine) resistance in Ghanaian malaria patients. American Journal of Tropical Medicine and Hygiene2001. 65 (3): 356.

 

BOOKS

1.      Towards Effective Disease Control in Ghana: Research and Policy Implications: Malaria. 2014. Edited by Kwadwo A. Koram, Collins K. Ahorlu, Michael D. Wilson, Dorothy Yeboah-Manu and Kwabena M. Bosompem.

2.      Towards Safe and Effective Treatment of Disease in Ghana: Contributions from Clinical Pharmacology. 2015. Edited by George Obeng Adjei and David Ofori-Adjei.

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